S-BIAD867alpha

Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting recombination (OME-NGFF)

Released 2023-09-01
Image Data Resource (IDR)

Content

  • 46 images
  • Study size: 15.2GiB
  • N files: 46
  • Filetype breakdown:
    • .ome.zarr.zip: 46 (15.2GiB)
OME-NGFF converted study from idr0004. To investigate the DNA damage response, we undertook a genome-wide study in Saccharomyces cerevisiae and identified 86 gene deletions that lead to increased levels of spontaneous Rad52 foci in proliferating diploid cells. More than half of the genes are conserved across species ranging from yeast to humans. Along with genes involved in DNA replication, repair, and chromatin remodeling, we found 22 previously uncharacterized open reading frames. Analysis of recombination rates and synthetic genetic interactions with rad52Delta suggests that multiple mechanisms are responsible for elevated levels of spontaneous Rad52 foci, including increased production of recombinogenic lesions, sister chromatid recombination defects, and improper focus assembly/disassembly. Our cell biological approach demonstrates the diversity of processes that converge on homologous recombination, protect against spontaneous DNA damage, and facilitate efficient repair.

Study information

Organism
Saccharomyces cerevisiae (brewer's yeast)
Imaging type
fluorescence microscopy
License
CC BY-NC-SA 3.0

Viewable images

Image ID Preview Filename Dimensions
(T, C, Z, Y, X)		 Download Size Actions
IM1 idr0004/P170.ome.zarr.zip None 451.3MiB
IM2 idr0004/P144.ome.zarr.zip None 455.7MiB
IM3 idr0004/P145.ome.zarr.zip None 442.1MiB
IM4 idr0004/P111.ome.zarr.zip None 379.9MiB
IM5 idr0004/P120.ome.zarr.zip None 363.5MiB
IM6 idr0004/P101.ome.zarr.zip None 305.0MiB
IM7 idr0004/P105.ome.zarr.zip None 370.1MiB
IM8 idr0004/P142.ome.zarr.zip None 402.5MiB
IM9 idr0004/P149.ome.zarr.zip None 439.8MiB
IM10 idr0004/P138.ome.zarr.zip None 213.7MiB
IM11 idr0004/P128.ome.zarr.zip None 207.3MiB
IM12 idr0004/P115.ome.zarr.zip None 391.1MiB
IM14 idr0004/P109.ome.zarr.zip None 394.4MiB
IM15 idr0004/P112.ome.zarr.zip None 140.2MiB
IM16 idr0004/P121.ome.zarr.zip None 329.0MiB
IM17 idr0004/P117.ome.zarr.zip None 221.1MiB
IM18 idr0004/P133.ome.zarr.zip None 358.0MiB
IM19 idr0004/P140.ome.zarr.zip None 224.8MiB
IM20 idr0004/P110.ome.zarr.zip None 405.8MiB
IM21 idr0004/P129.ome.zarr.zip None 281.3MiB
IM22 idr0004/P134.ome.zarr.zip None 327.9MiB
IM13 idr0004/P132.ome.zarr.zip None 364.3MiB
IM23 idr0004/P139.ome.zarr.zip None 345.8MiB
IM24 idr0004/P146.ome.zarr.zip None 390.1MiB
IM25 idr0004/P148.ome.zarr.zip None 430.7MiB
IM26 idr0004/P130.ome.zarr.zip None 287.7MiB
IM27 idr0004/P119.ome.zarr.zip None 372.2MiB
IM28 idr0004/P150.ome.zarr.zip None 296.6MiB
IM29 idr0004/P118.ome.zarr.zip None 191.5MiB
IM30 idr0004/P126.ome.zarr.zip None 337.0MiB
IM31 idr0004/P135.ome.zarr.zip None 386.5MiB
IM32 idr0004/P136.ome.zarr.zip None 398.9MiB
IM33 idr0004/P147.ome.zarr.zip None 337.1MiB
IM34 idr0004/P108.ome.zarr.zip None 416.7MiB
IM35 idr0004/P131.ome.zarr.zip None 424.4MiB
IM36 idr0004/P107.ome.zarr.zip None 426.8MiB
IM37 idr0004/P143.ome.zarr.zip None 416.1MiB
IM38 idr0004/P125.ome.zarr.zip None 304.7MiB
IM39 idr0004/P171.ome.zarr.zip None 280.1MiB
IM40 idr0004/P123.ome.zarr.zip None 249.7MiB
IM41 idr0004/P102.ome.zarr.zip None 192.2MiB
IM42 idr0004/P124.ome.zarr.zip None 194.8MiB
IM43 idr0004/P141.ome.zarr.zip None 375.6MiB
IM44 idr0004/P137.ome.zarr.zip None 421.5MiB
IM45 idr0004/P106.ome.zarr.zip None 361.8MiB
IM46 idr0004/P127.ome.zarr.zip None 261.3MiB

All images

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Image ID Filename Download Size Actions
IM1 idr0004/P170.ome.zarr.zip 451.3MiB Download
IM2 idr0004/P144.ome.zarr.zip 455.7MiB Download
IM3 idr0004/P145.ome.zarr.zip 442.1MiB Download
IM4 idr0004/P111.ome.zarr.zip 379.9MiB Download
IM5 idr0004/P120.ome.zarr.zip 363.5MiB Download
IM6 idr0004/P101.ome.zarr.zip 305.0MiB Download
IM7 idr0004/P105.ome.zarr.zip 370.1MiB Download
IM8 idr0004/P142.ome.zarr.zip 402.5MiB Download
IM9 idr0004/P149.ome.zarr.zip 439.8MiB Download
IM10 idr0004/P138.ome.zarr.zip 213.7MiB Download
IM11 idr0004/P128.ome.zarr.zip 207.3MiB Download
IM12 idr0004/P115.ome.zarr.zip 391.1MiB Download
IM14 idr0004/P109.ome.zarr.zip 394.4MiB Download
IM15 idr0004/P112.ome.zarr.zip 140.2MiB Download
IM16 idr0004/P121.ome.zarr.zip 329.0MiB Download
IM17 idr0004/P117.ome.zarr.zip 221.1MiB Download
IM18 idr0004/P133.ome.zarr.zip 358.0MiB Download
IM19 idr0004/P140.ome.zarr.zip 224.8MiB Download
IM20 idr0004/P110.ome.zarr.zip 405.8MiB Download
IM21 idr0004/P129.ome.zarr.zip 281.3MiB Download
IM22 idr0004/P134.ome.zarr.zip 327.9MiB Download
IM13 idr0004/P132.ome.zarr.zip 364.3MiB Download
IM23 idr0004/P139.ome.zarr.zip 345.8MiB Download
IM24 idr0004/P146.ome.zarr.zip 390.1MiB Download
IM25 idr0004/P148.ome.zarr.zip 430.7MiB Download
IM26 idr0004/P130.ome.zarr.zip 287.7MiB Download
IM27 idr0004/P119.ome.zarr.zip 372.2MiB Download
IM28 idr0004/P150.ome.zarr.zip 296.6MiB Download
IM29 idr0004/P118.ome.zarr.zip 191.5MiB Download
IM30 idr0004/P126.ome.zarr.zip 337.0MiB Download
IM31 idr0004/P135.ome.zarr.zip 386.5MiB Download
IM32 idr0004/P136.ome.zarr.zip 398.9MiB Download
IM33 idr0004/P147.ome.zarr.zip 337.1MiB Download
IM34 idr0004/P108.ome.zarr.zip 416.7MiB Download
IM35 idr0004/P131.ome.zarr.zip 424.4MiB Download
IM36 idr0004/P107.ome.zarr.zip 426.8MiB Download
IM37 idr0004/P143.ome.zarr.zip 416.1MiB Download
IM38 idr0004/P125.ome.zarr.zip 304.7MiB Download
IM39 idr0004/P171.ome.zarr.zip 280.1MiB Download
IM40 idr0004/P123.ome.zarr.zip 249.7MiB Download
IM41 idr0004/P102.ome.zarr.zip 192.2MiB Download
IM42 idr0004/P124.ome.zarr.zip 194.8MiB Download
IM43 idr0004/P141.ome.zarr.zip 375.6MiB Download
IM44 idr0004/P137.ome.zarr.zip 421.5MiB Download
IM45 idr0004/P106.ome.zarr.zip 361.8MiB Download
IM46 idr0004/P127.ome.zarr.zip 261.3MiB Download