S-BIAD882alpha

A machine learning approach to define antimalarial drug action from heterogeneous cell-based screens (OME-NGFF)

Released 2023-09-05
Image Data Resource (IDR)

Content

  • 22 images
  • Study size: 14.9TiB
  • N files: 163
  • Filetype breakdown:
    • .001: 22 (2.1TiB)
    • .002: 22 (2.1TiB)
    • .003: 22 (2.1TiB)
    • .004: 20 (2.0TiB)
    • .005: 20 (1.7TiB)
    • .006: 16 (1.5TiB)
    • .007: 14 (1.1TiB)
    • .008: 7 (600.5GiB)
    • .009: 6 (446.3GiB)
    • .010: 3 (230.2GiB)
    • .011: 2 (162.2GiB)
    • .012: 1 (100.0GiB)
    • .013: 1 (100.0GiB)
    • .014: 1 (100.0GiB)
    • .015: 1 (100.0GiB)
    • .016: 1 (100.0GiB)
    • .017: 1 (100.0GiB)
    • .018: 1 (100.0GiB)
    • .019: 1 (100.0GiB)
    • .020: 1 (45.2GiB)
OME-NGFF converted study from idr0090. Drug resistance threatens the effective prevention and treatment of an ever-increasing range of human infections. This highlights an urgent need for new and improved drugs with novel mechanisms of action to avoid cross-resistance. Current cell-based drug screens are, however, restricted to binary live/dead readouts with no provision for mechanism of action prediction. Machine learning methods are increasingly being used to improve information extraction from imaging data. Such methods, however, work poorly with heterogeneous cellular phenotypes and generally require time-consuming human-led training. We have developed a semi-supervised machine learning approach, combining human- and machine-labelled training data from mixed human malaria parasite cultures. Designed for high-throughput and high-resolution screening, our semi-supervised approach is robust to natural parasite morphological heterogeneity and correctly orders parasite developmental stages. Our approach also reproducibly detects and clusters drug-induced morphological outliers by mechanism of action, demonstrating the potential power of machine learning for accelerating cell-based drug discovery.

Study information

Organism
Plasmodium falciparum 3D7
Imaging type
fluorescence microscopy
License
CC BY 4.0

Viewable images

Image ID Preview Filename Dimensions
(T, C, Z, Y, X)		 Download Size Actions
IM1 idr0090/190710.ome.zarr.zip None 100.0GiB
IM2 idr0090/190206.ome.zarr.zip None 100.0GiB
IM3 idr0090/190614.ome.zarr.zip None 100.0GiB
IM4 idr0090/190607.ome.zarr.zip None 100.0GiB
IM5 idr0090/190211.ome.zarr.zip None 100.0GiB
IM6 idr0090/190322.ome.zarr.zip None 100.0GiB
IM7 idr0090/190705.ome.zarr.zip None 100.0GiB
IM8 idr0090/190306.ome.zarr.zip None 100.0GiB
IM9 idr0090/190809.ome.zarr.zip None 100.0GiB
IM10 idr0090/190502.ome.zarr.zip None 100.0GiB
IM11 idr0090/190528.ome.zarr.zip None 100.0GiB
IM12 idr0090/190628.ome.zarr.zip None 100.0GiB
IM13 idr0090/190327.ome.zarr.zip None 100.0GiB
IM14 idr0090/190531.ome.zarr.zip None 100.0GiB
IM15 idr0090/190904.ome.zarr.zip None 100.0GiB
IM18 idr0090/190213.ome.zarr.zip None 100.0GiB
IM19 idr0090/190621.ome.zarr.zip None 100.0GiB
IM20 idr0090/190510.ome.zarr.zip None 100.0GiB
IM21 idr0090/190220.ome.zarr.zip None 100.0GiB
IM22 idr0090/190313.ome.zarr.zip None 100.0GiB
IM16 idr0090/190129.ome.zarr.zip None 100.0GiB
IM17 idr0090/190227.ome.zarr.zip None 100.0GiB

All images

<
Image ID Filename Download Size Actions
IM1 idr0090/190710.ome.zarr.zip 100.0GiB Download
IM2 idr0090/190206.ome.zarr.zip 100.0GiB Download
IM3 idr0090/190614.ome.zarr.zip 100.0GiB Download
IM4 idr0090/190607.ome.zarr.zip 100.0GiB Download
IM5 idr0090/190211.ome.zarr.zip 100.0GiB Download
IM6 idr0090/190322.ome.zarr.zip 100.0GiB Download
IM7 idr0090/190705.ome.zarr.zip 100.0GiB Download
IM8 idr0090/190306.ome.zarr.zip 100.0GiB Download
IM9 idr0090/190809.ome.zarr.zip 100.0GiB Download
IM10 idr0090/190502.ome.zarr.zip 100.0GiB Download
IM11 idr0090/190528.ome.zarr.zip 100.0GiB Download
IM12 idr0090/190628.ome.zarr.zip 100.0GiB Download
IM13 idr0090/190327.ome.zarr.zip 100.0GiB Download
IM14 idr0090/190531.ome.zarr.zip 100.0GiB Download
IM15 idr0090/190904.ome.zarr.zip 100.0GiB Download
IM18 idr0090/190213.ome.zarr.zip 100.0GiB Download
IM19 idr0090/190621.ome.zarr.zip 100.0GiB Download
IM20 idr0090/190510.ome.zarr.zip 100.0GiB Download
IM21 idr0090/190220.ome.zarr.zip 100.0GiB Download
IM22 idr0090/190313.ome.zarr.zip 100.0GiB Download
IM16 idr0090/190129.ome.zarr.zip 100.0GiB Download
IM17 idr0090/190227.ome.zarr.zip 100.0GiB Download