S-BIAD885alpha

High content screen for genes whose downregulation by RNAi affects acccumulation of p53-binding protein 1 (53BP1) at sites of double strand breaks (OME-NGFF)

Released 2023-09-06
Image Data Resource (IDR)

Content

  • 147 images
  • Study size: 65.5GiB
  • N files (exc zip contents): 147
  • N files (inc zip contents): 147
  • Filetype breakdown:
    • .ome.zarr.zip: 147 (65.5GiB)
OME-NGFF converted study from idr0010. DNA double-strand breaks (DSBs) not only interrupt the genetic information, but also disrupt the chromatin structure, and both impairments require repair mechanisms to ensure genome integrity. We showed previously that RNF8-mediated chromatin ubiquitylation protects genome integrity by promoting the accumulation of repair factors at DSBs. Here, we provide evidence that, while RNF8 is necessary to trigger the DSB-associated ubiquitylations, it is not sufficient to sustain conjugated ubiquitin in this compartment. We identified RNF168 as a novel chromatin-associated ubiquitin ligase with an ability to bind ubiquitin. We show that RNF168 interacts with ubiquitylated H2A, assembles at DSBs in an RNF8-dependent manner, and, by targeting H2A and H2AX, amplifies local concentration of lysine 63-linked ubiquitin conjugates to the threshold required for retention of 53BP1 and BRCA1. Thus, RNF168 defines a new pathway involving sequential ubiquitylations on damaged chromosomes and uncovers a functional cooperation between E3 ligases in genome maintenance.

Study information

Organism
Homo sapiens (human)
Study type
Imaging type
fluorescence microscopy

Viewable images

Image ID Preview Filename Dimensions
(T, C, Z, Y, X)		 Download Size Actions
IM1 idr0010/76-45.ome.zarr.zip None 451.3MiB
IM2 idr0010/65-12.ome.zarr.zip None 455.6MiB
IM3 idr0010/34-30.ome.zarr.zip None 458.9MiB
IM4 idr0010/124-44.ome.zarr.zip None 464.5MiB
IM5 idr0010/47-35.ome.zarr.zip None 439.4MiB
IM6 idr0010/155-45.ome.zarr.zip None 457.4MiB
IM7 idr0010/20-29.ome.zarr.zip None 446.2MiB
IM8 idr0010/16-45.ome.zarr.zip None 465.7MiB
IM9 idr0010/33-46.ome.zarr.zip None 464.3MiB
IM10 idr0010/131-20.ome.zarr.zip None 458.1MiB
IM11 idr0010/74-26.ome.zarr.zip None 449.5MiB
IM12 idr0010/14-35.ome.zarr.zip None 441.3MiB
IM13 idr0010/108-24.ome.zarr.zip None 468.4MiB
IM14 idr0010/48-29.ome.zarr.zip None 444.9MiB
IM15 idr0010/63-27.ome.zarr.zip None 474.1MiB
IM16 idr0010/151-48.ome.zarr.zip None 432.5MiB
IM17 idr0010/19-29.ome.zarr.zip None 450.4MiB
IM18 idr0010/46-33.ome.zarr.zip None 434.1MiB
IM19 idr0010/129-58.ome.zarr.zip None 440.5MiB
IM20 idr0010/13-3.ome.zarr.zip None 453.8MiB
IM21 idr0010/125-27.ome.zarr.zip None 450.9MiB
IM22 idr0010/103.ome.zarr.zip None 460.3MiB
IM23 idr0010/146-35.ome.zarr.zip None 458.9MiB
IM24 idr0010/44-13.ome.zarr.zip None 435.3MiB
IM25 idr0010/152-14.ome.zarr.zip None 462.3MiB
IM26 idr0010/88-40.ome.zarr.zip None 439.5MiB
IM27 idr0010/10-34.ome.zarr.zip None 454.1MiB
IM28 idr0010/133-29.ome.zarr.zip None 452.8MiB
IM29 idr0010/17-43.ome.zarr.zip None 439.5MiB
IM30 idr0010/82-14.ome.zarr.zip None 462.6MiB
IM31 idr0010/85.ome.zarr.zip None 459.7MiB
IM32 idr0010/93-40.ome.zarr.zip None 450.4MiB
IM33 idr0010/110-35.ome.zarr.zip None 474.5MiB
IM34 idr0010/130-16.ome.zarr.zip None 460.2MiB
IM35 idr0010/136-19.ome.zarr.zip None 459.8MiB
IM36 idr0010/87-48.ome.zarr.zip None 458.1MiB
IM37 idr0010/140-35.ome.zarr.zip None 435.2MiB
IM38 idr0010/80-29.ome.zarr.zip None 453.5MiB
IM39 idr0010/12-23.ome.zarr.zip None 454.5MiB
IM40 idr0010/23-15.ome.zarr.zip None 457.6MiB
IM41 idr0010/68-30.ome.zarr.zip None 428.9MiB
IM42 idr0010/72-33.ome.zarr.zip None 471.0MiB
IM43 idr0010/36-28.ome.zarr.zip None 448.5MiB
IM44 idr0010/147-09.ome.zarr.zip None 464.4MiB
IM45 idr0010/7-14.ome.zarr.zip None 453.5MiB
IM46 idr0010/101-24.ome.zarr.zip None 456.2MiB
IM47 idr0010/97.ome.zarr.zip None 452.5MiB
IM48 idr0010/39-44.ome.zarr.zip None 470.9MiB
IM49 idr0010/40-37.ome.zarr.zip None 433.8MiB
IM50 idr0010/120.ome.zarr.zip None 470.8MiB
IM51 idr0010/115-11.ome.zarr.zip None 476.0MiB
IM52 idr0010/11-08.ome.zarr.zip None 466.4MiB
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IM54 idr0010/139-29.ome.zarr.zip None 461.6MiB
IM55 idr0010/79-39.ome.zarr.zip None 450.4MiB
IM56 idr0010/99.ome.zarr.zip None 446.8MiB
IM57 idr0010/159-13.ome.zarr.zip None 461.1MiB
IM58 idr0010/2-60.ome.zarr.zip None 459.8MiB
IM59 idr0010/94-05.ome.zarr.zip None 446.7MiB
IM60 idr0010/92-44.ome.zarr.zip None 452.7MiB
IM61 idr0010/38-21.ome.zarr.zip None 474.3MiB
IM62 idr0010/4-36.ome.zarr.zip None 459.6MiB
IM63 idr0010/106-13.ome.zarr.zip None 448.8MiB
IM64 idr0010/132-19.ome.zarr.zip None 446.6MiB
IM65 idr0010/127-44.ome.zarr.zip None 457.9MiB
IM66 idr0010/37-34.ome.zarr.zip None 442.7MiB
IM67 idr0010/62-01.ome.zarr.zip None 468.2MiB
IM68 idr0010/148-48.ome.zarr.zip None 439.6MiB
IM69 idr0010/144-21.ome.zarr.zip None 456.7MiB
IM70 idr0010/73-40.ome.zarr.zip None 459.4MiB
IM71 idr0010/141-27.ome.zarr.zip None 439.2MiB
IM72 idr0010/28-43.ome.zarr.zip None 447.3MiB
IM73 idr0010/98-23.ome.zarr.zip None 473.2MiB
IM74 idr0010/113-34.ome.zarr.zip None 472.5MiB
IM75 idr0010/21-58.ome.zarr.zip None 459.9MiB
IM76 idr0010/24-27.ome.zarr.zip None 450.4MiB
IM77 idr0010/81-44.ome.zarr.zip None 463.1MiB
IM78 idr0010/42-44.ome.zarr.zip None 447.8MiB
IM79 idr0010/61-43.ome.zarr.zip None 470.9MiB
IM80 idr0010/91-29.ome.zarr.zip None 450.4MiB
IM81 idr0010/114-58.ome.zarr.zip None 481.1MiB
IM82 idr0010/154-43.ome.zarr.zip None 472.2MiB
IM83 idr0010/149-21.ome.zarr.zip None 451.6MiB
IM84 idr0010/83-48.ome.zarr.zip None 456.2MiB
IM85 idr0010/107.ome.zarr.zip None 472.5MiB
IM86 idr0010/15-11.ome.zarr.zip None 451.9MiB
IM87 idr0010/109-36.ome.zarr.zip None 446.6MiB
IM88 idr0010/45-31.ome.zarr.zip None 453.8MiB
IM89 idr0010/104-13.ome.zarr.zip None 473.5MiB
IM90 idr0010/121-11.ome.zarr.zip None 455.7MiB
IM91 idr0010/143-29.ome.zarr.zip None 430.1MiB
IM92 idr0010/41-31.ome.zarr.zip None 435.8MiB
IM93 idr0010/70-12.ome.zarr.zip None 461.1MiB
IM94 idr0010/134-34.ome.zarr.zip None 440.3MiB
IM95 idr0010/30-44.ome.zarr.zip None 460.7MiB
IM96 idr0010/145-20.ome.zarr.zip None 449.0MiB
IM97 idr0010/100-27.ome.zarr.zip None 442.3MiB
IM98 idr0010/3-11.ome.zarr.zip None 454.6MiB
IM99 idr0010/75-33.ome.zarr.zip None 436.9MiB
IM100 idr0010/156-42.ome.zarr.zip None 455.3MiB
IM101 idr0010/135-21.ome.zarr.zip None 460.4MiB
IM102 idr0010/118-33.ome.zarr.zip None 456.4MiB
IM103 idr0010/123-18.ome.zarr.zip None 480.0MiB
IM104 idr0010/102.ome.zarr.zip None 440.1MiB
IM105 idr0010/78-31.ome.zarr.zip None 460.7MiB
IM106 idr0010/84-33.ome.zarr.zip None 446.4MiB
IM107 idr0010/105-12.ome.zarr.zip None 464.3MiB
IM108 idr0010/26-41.ome.zarr.zip None 471.0MiB
IM109 idr0010/119-43.ome.zarr.zip None 461.1MiB
IM110 idr0010/158-10.ome.zarr.zip None 449.9MiB
IM111 idr0010/18-18.ome.zarr.zip None 450.9MiB
IM112 idr0010/128-35.ome.zarr.zip None 478.1MiB
IM113 idr0010/116-25.ome.zarr.zip None 483.2MiB
IM114 idr0010/49-06.ome.zarr.zip None 465.6MiB
IM115 idr0010/32-42.ome.zarr.zip None 447.8MiB
IM116 idr0010/64-41.ome.zarr.zip None 447.8MiB
IM117 idr0010/8-10.ome.zarr.zip None 451.4MiB
IM118 idr0010/112-02.ome.zarr.zip None 462.7MiB
IM119 idr0010/157-46.ome.zarr.zip None 465.7MiB
IM120 idr0010/27-37.ome.zarr.zip None 453.9MiB
IM121 idr0010/9-12.ome.zarr.zip None 452.7MiB
IM122 idr0010/89-16.ome.zarr.zip None 458.1MiB
IM123 idr0010/31-48.ome.zarr.zip None 468.0MiB
IM124 idr0010/60-30.ome.zarr.zip None 484.7MiB
IM125 idr0010/111-07.ome.zarr.zip None 464.9MiB
IM126 idr0010/126-12.ome.zarr.zip None 464.9MiB
IM127 idr0010/95-48.ome.zarr.zip None 438.2MiB
IM128 idr0010/22-21.ome.zarr.zip None 487.7MiB
IM129 idr0010/96-14.ome.zarr.zip None 446.8MiB
IM130 idr0010/25-28.ome.zarr.zip None 475.1MiB
IM131 idr0010/137-11.ome.zarr.zip None 452.7MiB
IM132 idr0010/142-29.ome.zarr.zip None 461.1MiB
IM133 idr0010/86-31.ome.zarr.zip None 451.5MiB
IM134 idr0010/90-27.ome.zarr.zip None 422.0MiB
IM135 idr0010/35-18.ome.zarr.zip None 460.6MiB
IM136 idr0010/1-23.ome.zarr.zip None 462.5MiB
IM137 idr0010/122-42.ome.zarr.zip None 475.6MiB
IM138 idr0010/69-49.ome.zarr.zip None 456.7MiB
IM139 idr0010/71-19.ome.zarr.zip None 460.5MiB
IM140 idr0010/117-12.ome.zarr.zip None 459.5MiB
IM141 idr0010/138-27.ome.zarr.zip None 459.3MiB
IM142 idr0010/67-44.ome.zarr.zip None 470.7MiB
IM143 idr0010/29-30.ome.zarr.zip None 437.4MiB
IM144 idr0010/150-15.ome.zarr.zip None 451.4MiB
IM145 idr0010/43-04.ome.zarr.zip None 472.2MiB
IM146 idr0010/66-37.ome.zarr.zip None 455.3MiB
IM147 idr0010/77-20.ome.zarr.zip None 459.6MiB

All images

Image ID Filename Download Size Actions
IM1 idr0010/76-45.ome.zarr.zip 451.3MiB Download
IM2 idr0010/65-12.ome.zarr.zip 455.6MiB Download
IM3 idr0010/34-30.ome.zarr.zip 458.9MiB Download
IM4 idr0010/124-44.ome.zarr.zip 464.5MiB Download
IM5 idr0010/47-35.ome.zarr.zip 439.4MiB Download
IM6 idr0010/155-45.ome.zarr.zip 457.4MiB Download
IM7 idr0010/20-29.ome.zarr.zip 446.2MiB Download
IM8 idr0010/16-45.ome.zarr.zip 465.7MiB Download
IM9 idr0010/33-46.ome.zarr.zip 464.3MiB Download
IM10 idr0010/131-20.ome.zarr.zip 458.1MiB Download
IM11 idr0010/74-26.ome.zarr.zip 449.5MiB Download
IM12 idr0010/14-35.ome.zarr.zip 441.3MiB Download
IM13 idr0010/108-24.ome.zarr.zip 468.4MiB Download
IM14 idr0010/48-29.ome.zarr.zip 444.9MiB Download
IM15 idr0010/63-27.ome.zarr.zip 474.1MiB Download
IM16 idr0010/151-48.ome.zarr.zip 432.5MiB Download
IM17 idr0010/19-29.ome.zarr.zip 450.4MiB Download
IM18 idr0010/46-33.ome.zarr.zip 434.1MiB Download
IM19 idr0010/129-58.ome.zarr.zip 440.5MiB Download
IM20 idr0010/13-3.ome.zarr.zip 453.8MiB Download
IM21 idr0010/125-27.ome.zarr.zip 450.9MiB Download
IM22 idr0010/103.ome.zarr.zip 460.3MiB Download
IM23 idr0010/146-35.ome.zarr.zip 458.9MiB Download
IM24 idr0010/44-13.ome.zarr.zip 435.3MiB Download
IM25 idr0010/152-14.ome.zarr.zip 462.3MiB Download
IM26 idr0010/88-40.ome.zarr.zip 439.5MiB Download
IM27 idr0010/10-34.ome.zarr.zip 454.1MiB Download
IM28 idr0010/133-29.ome.zarr.zip 452.8MiB Download
IM29 idr0010/17-43.ome.zarr.zip 439.5MiB Download
IM30 idr0010/82-14.ome.zarr.zip 462.6MiB Download
IM31 idr0010/85.ome.zarr.zip 459.7MiB Download
IM32 idr0010/93-40.ome.zarr.zip 450.4MiB Download
IM33 idr0010/110-35.ome.zarr.zip 474.5MiB Download
IM34 idr0010/130-16.ome.zarr.zip 460.2MiB Download
IM35 idr0010/136-19.ome.zarr.zip 459.8MiB Download
IM36 idr0010/87-48.ome.zarr.zip 458.1MiB Download
IM37 idr0010/140-35.ome.zarr.zip 435.2MiB Download
IM38 idr0010/80-29.ome.zarr.zip 453.5MiB Download
IM39 idr0010/12-23.ome.zarr.zip 454.5MiB Download
IM40 idr0010/23-15.ome.zarr.zip 457.6MiB Download
IM41 idr0010/68-30.ome.zarr.zip 428.9MiB Download
IM42 idr0010/72-33.ome.zarr.zip 471.0MiB Download
IM43 idr0010/36-28.ome.zarr.zip 448.5MiB Download
IM44 idr0010/147-09.ome.zarr.zip 464.4MiB Download
IM45 idr0010/7-14.ome.zarr.zip 453.5MiB Download
IM46 idr0010/101-24.ome.zarr.zip 456.2MiB Download
IM47 idr0010/97.ome.zarr.zip 452.5MiB Download
IM48 idr0010/39-44.ome.zarr.zip 470.9MiB Download
IM49 idr0010/40-37.ome.zarr.zip 433.8MiB Download
IM50 idr0010/120.ome.zarr.zip 470.8MiB Download
IM51 idr0010/115-11.ome.zarr.zip 476.0MiB Download
IM52 idr0010/11-08.ome.zarr.zip 466.4MiB Download
IM53 idr0010/6-14.ome.zarr.zip 477.0MiB Download
IM54 idr0010/139-29.ome.zarr.zip 461.6MiB Download
IM55 idr0010/79-39.ome.zarr.zip 450.4MiB Download
IM56 idr0010/99.ome.zarr.zip 446.8MiB Download
IM57 idr0010/159-13.ome.zarr.zip 461.1MiB Download
IM58 idr0010/2-60.ome.zarr.zip 459.8MiB Download
IM59 idr0010/94-05.ome.zarr.zip 446.7MiB Download
IM60 idr0010/92-44.ome.zarr.zip 452.7MiB Download
IM61 idr0010/38-21.ome.zarr.zip 474.3MiB Download
IM62 idr0010/4-36.ome.zarr.zip 459.6MiB Download
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IM65 idr0010/127-44.ome.zarr.zip 457.9MiB Download
IM66 idr0010/37-34.ome.zarr.zip 442.7MiB Download
IM67 idr0010/62-01.ome.zarr.zip 468.2MiB Download
IM68 idr0010/148-48.ome.zarr.zip 439.6MiB Download
IM69 idr0010/144-21.ome.zarr.zip 456.7MiB Download
IM70 idr0010/73-40.ome.zarr.zip 459.4MiB Download
IM71 idr0010/141-27.ome.zarr.zip 439.2MiB Download
IM72 idr0010/28-43.ome.zarr.zip 447.3MiB Download
IM73 idr0010/98-23.ome.zarr.zip 473.2MiB Download
IM74 idr0010/113-34.ome.zarr.zip 472.5MiB Download
IM75 idr0010/21-58.ome.zarr.zip 459.9MiB Download
IM76 idr0010/24-27.ome.zarr.zip 450.4MiB Download
IM77 idr0010/81-44.ome.zarr.zip 463.1MiB Download
IM78 idr0010/42-44.ome.zarr.zip 447.8MiB Download
IM79 idr0010/61-43.ome.zarr.zip 470.9MiB Download
IM80 idr0010/91-29.ome.zarr.zip 450.4MiB Download
IM81 idr0010/114-58.ome.zarr.zip 481.1MiB Download
IM82 idr0010/154-43.ome.zarr.zip 472.2MiB Download
IM83 idr0010/149-21.ome.zarr.zip 451.6MiB Download
IM84 idr0010/83-48.ome.zarr.zip 456.2MiB Download
IM85 idr0010/107.ome.zarr.zip 472.5MiB Download
IM86 idr0010/15-11.ome.zarr.zip 451.9MiB Download
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IM88 idr0010/45-31.ome.zarr.zip 453.8MiB Download
IM89 idr0010/104-13.ome.zarr.zip 473.5MiB Download
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IM91 idr0010/143-29.ome.zarr.zip 430.1MiB Download
IM92 idr0010/41-31.ome.zarr.zip 435.8MiB Download
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IM94 idr0010/134-34.ome.zarr.zip 440.3MiB Download
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IM96 idr0010/145-20.ome.zarr.zip 449.0MiB Download
IM97 idr0010/100-27.ome.zarr.zip 442.3MiB Download
IM98 idr0010/3-11.ome.zarr.zip 454.6MiB Download
IM99 idr0010/75-33.ome.zarr.zip 436.9MiB Download
IM100 idr0010/156-42.ome.zarr.zip 455.3MiB Download
IM101 idr0010/135-21.ome.zarr.zip 460.4MiB Download
IM102 idr0010/118-33.ome.zarr.zip 456.4MiB Download
IM103 idr0010/123-18.ome.zarr.zip 480.0MiB Download
IM104 idr0010/102.ome.zarr.zip 440.1MiB Download
IM105 idr0010/78-31.ome.zarr.zip 460.7MiB Download
IM106 idr0010/84-33.ome.zarr.zip 446.4MiB Download
IM107 idr0010/105-12.ome.zarr.zip 464.3MiB Download
IM108 idr0010/26-41.ome.zarr.zip 471.0MiB Download
IM109 idr0010/119-43.ome.zarr.zip 461.1MiB Download
IM110 idr0010/158-10.ome.zarr.zip 449.9MiB Download
IM111 idr0010/18-18.ome.zarr.zip 450.9MiB Download
IM112 idr0010/128-35.ome.zarr.zip 478.1MiB Download
IM113 idr0010/116-25.ome.zarr.zip 483.2MiB Download
IM114 idr0010/49-06.ome.zarr.zip 465.6MiB Download
IM115 idr0010/32-42.ome.zarr.zip 447.8MiB Download
IM116 idr0010/64-41.ome.zarr.zip 447.8MiB Download
IM117 idr0010/8-10.ome.zarr.zip 451.4MiB Download
IM118 idr0010/112-02.ome.zarr.zip 462.7MiB Download
IM119 idr0010/157-46.ome.zarr.zip 465.7MiB Download
IM120 idr0010/27-37.ome.zarr.zip 453.9MiB Download
IM121 idr0010/9-12.ome.zarr.zip 452.7MiB Download
IM122 idr0010/89-16.ome.zarr.zip 458.1MiB Download
IM123 idr0010/31-48.ome.zarr.zip 468.0MiB Download
IM124 idr0010/60-30.ome.zarr.zip 484.7MiB Download
IM125 idr0010/111-07.ome.zarr.zip 464.9MiB Download
IM126 idr0010/126-12.ome.zarr.zip 464.9MiB Download
IM127 idr0010/95-48.ome.zarr.zip 438.2MiB Download
IM128 idr0010/22-21.ome.zarr.zip 487.7MiB Download
IM129 idr0010/96-14.ome.zarr.zip 446.8MiB Download
IM130 idr0010/25-28.ome.zarr.zip 475.1MiB Download
IM131 idr0010/137-11.ome.zarr.zip 452.7MiB Download
IM132 idr0010/142-29.ome.zarr.zip 461.1MiB Download
IM133 idr0010/86-31.ome.zarr.zip 451.5MiB Download
IM134 idr0010/90-27.ome.zarr.zip 422.0MiB Download
IM135 idr0010/35-18.ome.zarr.zip 460.6MiB Download
IM136 idr0010/1-23.ome.zarr.zip 462.5MiB Download
IM137 idr0010/122-42.ome.zarr.zip 475.6MiB Download
IM138 idr0010/69-49.ome.zarr.zip 456.7MiB Download
IM139 idr0010/71-19.ome.zarr.zip 460.5MiB Download
IM140 idr0010/117-12.ome.zarr.zip 459.5MiB Download
IM141 idr0010/138-27.ome.zarr.zip 459.3MiB Download
IM142 idr0010/67-44.ome.zarr.zip 470.7MiB Download
IM143 idr0010/29-30.ome.zarr.zip 437.4MiB Download
IM144 idr0010/150-15.ome.zarr.zip 451.4MiB Download
IM145 idr0010/43-04.ome.zarr.zip 472.2MiB Download
IM146 idr0010/66-37.ome.zarr.zip 455.3MiB Download
IM147 idr0010/77-20.ome.zarr.zip 459.6MiB Download